AbSeq Protocol Using the Nano-Well Cartridge-Based Rhapsody Platform to Generate Protein and Transcript Expression Data on the Single-Cell Level

By including oligonucleotide-labeled antibodies into high-throughput single-cell RNA-sequencing protocols, combined transcript and protein expression data can be acquired on the single-cell level. Here, we describe a protocol for the combined analysis of over 40 proteins and 400 genes on over 10(4)...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Erickson, Jami R., Mair, Florian, Bugos, Grace, Martin, Jody, Tyznik, Aaron J., Nakamoto, Margaret, Mortimer, Stefanie, Prlic, Martin
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Protocol
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7523635/
https://ncbi.nlm.nih.gov/pubmed/33000001
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100092
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