"Sample pooling of RNA extracts to speed up SARS-CoV-2 diagnosis using CDC FDA EUA RT-qPCR kit"

BACKGROUND: The CDC protocol for SARS-CoV2 RT-PCR diagnosis (2019-nCoV CDC kit) is considered a gold standard worldwide; based on three different FAM probes (N1 and N2 for viral detection; RP for RNA extraction quality control), three reactions per sample are needed for SARS-CoV-2 diagnosis. RESULTS...

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Enregistré dans:
Détails bibliographiques
Publié dans:Virus Res
Auteurs principaux: Freire-Paspuel, Byron, Vega-Mariño, Patricio, Velez, Alberto, Cruz, Marilyn, Garcia-Bereguiain, Miguel Angel
Format: Artigo
Langue:Inglês
Publié: Elsevier B.V. 2020
Sujets:
Short Communication
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7513759/
https://ncbi.nlm.nih.gov/pubmed/32979475
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.virusres.2020.198173
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