EDTA-FISH: A Simple and Effective Approach to Reduce Non-specific Adsorption of Probes in Fluorescence in situ Hybridization (FISH) for Environmental Samples

Fluorescence in situ hybridization (FISH) is a widely used molecular technique in microbial ecology. However, the non-specific adsorption of fluorescent probes and resulting high intensity of background signals from mineral particles hampers the specific detection of microbial cells in grain-rich en...

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Enregistré dans:
Détails bibliographiques
Publié dans:Microbes Environ
Auteurs principaux: Morono, Yuki, Kubota, Kengo, Tsukagoshi, Daisuke, Terada, Takeshi
Format: Artigo
Langue:Inglês
Publié: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2020
Sujets:
Short Communication
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7511785/
https://ncbi.nlm.nih.gov/pubmed/32595184
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1264/jsme2.ME20062
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