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Interaction of Cucurbit[7]uril With Protease Substrates: Application to Nanosecond Time-Resolved Fluorescence Assays

We report the use of the macrocyclic host cucurbit[7]uril (CB7) as a supramolecular additive in nanosecond time-resolved fluorescence (Nano-TRF) assays for proteases to enhance the discrimination of substrates and products and, thereby, the sensitivity. A peptide substrate was labeled with 2,3-diaza...

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Detalhes bibliográficos
Publicado no:Front Chem
Main Authors: Hennig, Andreas, Nau, Werner M.
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7511663/
https://ncbi.nlm.nih.gov/pubmed/33134264
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fchem.2020.00806
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