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Biological observations in microbiota analysis are robust to the choice of 16S rRNA gene sequencing processing algorithm: case study on human milk microbiota

BACKGROUND: In recent years, the microbiome field has undergone a shift from clustering-based methods of operational taxonomic unit (OTU) designation based on sequence similarity to denoising algorithms that identify exact amplicon sequence variants (ASVs), and methods to identify contaminating bact...

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Detalhes bibliográficos
Publicado no:BMC Microbiol
Main Authors: Moossavi, Shirin, Atakora, Faisal, Fehr, Kelsey, Khafipour, Ehsan
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7501722/
https://ncbi.nlm.nih.gov/pubmed/32948144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12866-020-01949-7
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