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Bacterial Expression, Purification, and Initial Characterization of a Full-length Cas13b Protein from Porphyromonas gingivalis
The CRISPR-Cas13b system is a recently identified Class 2, RNA-targeting CRISPR-Cas system. The system has been repurposed to achieve robust mRNA knockdown and precise RNA-editing in mammalian cells. While the CRISPR-Cas13b system has become a powerful tool for nucleic acids manipulation, the mechan...
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| Foilsithe in: | Protein Expr Purif |
|---|---|
| Main Authors: | , , , |
| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
2020
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7495503/ https://ncbi.nlm.nih.gov/pubmed/32006655 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2020.105588 |
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