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Two-step method for isolation of high-quality RNA from stored seeds of maize rich in starch
A modified SDS–Trizol method was optimized for isolation of total RNA from the stored maize seeds at regular interval of one month for 4 months. Use of SDS extraction buffer before the use of Trizol reduced the co-precipitation problem associated with high carbohydrate content in the seed. Recorded...
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| Publicado no: | 3 Biotech |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer International Publishing
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7494719/ https://ncbi.nlm.nih.gov/pubmed/32999811 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-020-02424-w |
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