Two-step method for isolation of high-quality RNA from stored seeds of maize rich in starch

A modified SDS–Trizol method was optimized for isolation of total RNA from the stored maize seeds at regular interval of one month for 4 months. Use of SDS extraction buffer before the use of Trizol reduced the co-precipitation problem associated with high carbohydrate content in the seed. Recorded...

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Vydáno v:3 Biotech
Hlavní autoři: Dutta, Suman, Muthusamy, Vignesh, Chhabra, Rashmi, Zunjare, Rajkumar U., Hossain, Firoz
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer International Publishing 2020
Témata:
Protocols and Methods
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7494719/
https://ncbi.nlm.nih.gov/pubmed/32999811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-020-02424-w
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