Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

BACKGROUND: To date, CRISPR/Cas9 RNP editing tools have not been applied to the genetic modification of banana. Here, the establishment of a PEG-mediated banana protoplast transformation system makes it possible to build an efficient DNA-free method for a site-directed mutagenesis system. RESULTS: P...

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Publicado en:BMC Plant Biol
Autores principales: Wu, Shaoping, Zhu, Haocheng, Liu, Jinxing, Yang, Qiaosong, Shao, Xiuhong, Bi, Fangcheng, Hu, Chunhua, Huo, Heqiang, Chen, Kunling, Yi, Ganjun
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2020
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7493974/
https://ncbi.nlm.nih.gov/pubmed/32933485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12870-020-02609-8
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