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Methylase-assisted subcloning for high throughput BioBrick assembly

The BioBrick standard makes possible iterated pairwise assembly of cloned parts without any depletion of unique restriction sites. Every part that conforms to the standard is compatible with every other part, thereby fostering a worldwide user community. The assembly methods, however, are labor inte...

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Bibliografiske detaljer
Udgivet i:PeerJ
Hovedforfatter: Matsumura, Ichiro
Format: Artigo
Sprog:Inglês
Udgivet: PeerJ Inc. 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7489255/
https://ncbi.nlm.nih.gov/pubmed/32974095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.9841
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