Development of a differential multiplex real-time PCR assay for porcine circovirus type 2 (PCV2) genotypes PCV2a, PCV2b and PCV2d

A multiplex quantitative real-time polymerase chain reaction (mqPCR) assay was developed and validated for detection and differentiation of porcine circovirus type 2 (PCV2) genotypes, PCV2a, PCV2b and PCV2d. Single nucleotide polymorphism in primers or probes was deployed for different genotype dete...

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Udgivet i:J Virol Methods
Main Authors: Wang, Yin, Noll, Lance, Porter, Elizabeth, Stoy, Colin, Dong, Junsheng, Anderson, Joe, Fu, Jinping, Pogranichniy, Roman, Woodworth, Jason, Peddireddi, Lalitha, Bai, Jianfa
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier B.V. 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7486290/
https://ncbi.nlm.nih.gov/pubmed/32926893
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2020.113971
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