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Multiplexed detection and isolation of viable low-frequency cytokine-secreting human B cells using cytokine secretion assay and flow cytometry (CSA-Flow)

The ability to functionally characterize cytokine-secreting immune cells has broad implications in both health and a range of immune-mediated and auto-immune diseases. Low-frequency cytokine-defined immune-cell subsets can play key immune-regulatory roles, yet their detailed study is often hampered...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Rezk, Ayman, Li, Rui, Bar-Or, Amit
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7481209/
https://ncbi.nlm.nih.gov/pubmed/32908164
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-71750-z
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