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Flow cytometry assay for the detection of single-copy DNA in human lymphocytes

Specific nucleic acid sequences can be detected in individual cells by in situ hybridization. However, when very few copies of a target sequence are present per cell, its signal is undetectable by flow cytometry. Although various approaches have been developed to increase fluorescence signals for in...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Uno, Naoki, Kaku, Norihito, Morinaga, Yoshitomo, Hasegawa, Hiroo, Yanagihara, Katsunori
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7470942/
https://ncbi.nlm.nih.gov/pubmed/32544240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa515
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