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Temporal and Concentration Effects of Methanol on Cryopreservation of Zebrafish (Danio rerio) Sperm

We evaluated the cryoprotective effects of methanol on zebrafish sperm at different concentrations, exposure times, and stages during cryopreservation. Samples were collected by crushing of dissected testes or abdominal stripping. After exposure to 0%, 2%, 5%, 8%, and 10% methanol for 0–11 min, fres...

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Bibliografiske detaljer
Udgivet i:Zebrafish
Main Authors: Yang, Huiping, Hu, E, Tiersch, Terrence, Carmichael, Carrie, Matthews, Jen, Varga, Zoltan M.
Format: Artigo
Sprog:Inglês
Udgivet: Mary Ann Liebert, Inc., publishers 2020
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Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7455470/
https://ncbi.nlm.nih.gov/pubmed/32598236
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/zeb.2019.1849
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