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Temporal and Concentration Effects of Methanol on Cryopreservation of Zebrafish (Danio rerio) Sperm

We evaluated the cryoprotective effects of methanol on zebrafish sperm at different concentrations, exposure times, and stages during cryopreservation. Samples were collected by crushing of dissected testes or abdominal stripping. After exposure to 0%, 2%, 5%, 8%, and 10% methanol for 0–11 min, fres...

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Detalhes bibliográficos
Publicado no:Zebrafish
Main Authors: Yang, Huiping, Hu, E, Tiersch, Terrence, Carmichael, Carrie, Matthews, Jen, Varga, Zoltan M.
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc., publishers 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7455470/
https://ncbi.nlm.nih.gov/pubmed/32598236
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/zeb.2019.1849
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