CRISPR/Cas with ribonucleoprotein complexes and transiently selected telomere vectors allows highly efficient marker-free and multiple genome editing in Botrytis cinerea

CRISPR/Cas has become the state-of-the-art technology for genetic manipulation in diverse organisms, enabling targeted genetic changes to be performed with unprecedented efficiency. Here we report on the first establishment of robust CRISPR/Cas editing in the important necrotrophic plant pathogen Bo...

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Bibliographische Detailangaben
Veröffentlicht in:PLoS Pathog
Hauptverfasser: Leisen, Thomas, Bietz, Fabian, Werner, Janina, Wegner, Alex, Schaffrath, Ulrich, Scheuring, David, Willmund, Felix, Mosbach, Andreas, Scalliet, Gabriel, Hahn, Matthias
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2020
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7451986/
https://ncbi.nlm.nih.gov/pubmed/32804988
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.ppat.1008326
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