Single-strand RPA for rapid and sensitive detection of SARS-CoV-2 RNA

We report the single-strand Recombinase Polymerase Amplification (ssRPA) method, which merges the fast, isothermal amplification of RPA with subsequent rapid conversion of the double-strand DNA amplicon to single strands, and hence enables facile hybridization-based, high-specificity readout. We dem...

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Foilsithe in:medRxiv
Main Authors: Kim, Youngeun, Yaseen, Adam B., Kishi, Jocelyn Y., Hong, Fan, Saka, Sinem K., Sheng, Kuanwei, Gopalkrishnan, Nikhil, Schaus, Thomas E., Yin, Peng
Formáid: Artigo
Teanga:Inglês
Foilsithe: Cold Spring Harbor Laboratory 2020
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Article
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7444299/
https://ncbi.nlm.nih.gov/pubmed/32839783
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/2020.08.17.20177006
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