Quantification of residual AEBSF-related impurities by reversed-phase liquid chromatography()

During research of a broadly neutralizing antibody (bNAb) for HIV-1 infection, site-specific clipping was observed during cell culture incubation. Protease inhibitor, 4-(2-aminoethyl) benzenesulfonyl fluoride (AEBSF), was supplemented to the cell culture feeding to mitigate clipping as one of the co...

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Detalhes bibliográficos
Publicado no:J Chromatogr B Analyt Technol Biomed Life Sci
Main Authors: Cai, Cindy X., Schneck, Nicole A., Harris, Doug, Blackstock, Daniel, Ivleva, Vera B., Cheng, Kuang-Chuan, Charlton, Adam, Arnold, Frank J., Cooper, Jonathan W., Lei, Q. Paula
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7439605/
https://ncbi.nlm.nih.gov/pubmed/30953918
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jchromb.2019.03.022
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