Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

Saturation mutagenesis constitutes a powerful method in the directed evolution of enzymes. Traditional protocols of whole plasmid amplification such as Stratagene’s QuikChange™ sometimes fail when the templates are difficult to amplify. In order to overcome such restrictions, we have devised a simpl...

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Vydáno v:Appl Microbiol Biotechnol
Hlavní autoři: Sanchis, Joaquin, Fernández, Layla, Carballeira, J. Daniel, Drone, Jullien, Gumulya, Yosephine, Höbenreich, Horst, Kahakeaw, Daniel, Kille, Sabrina, Lohmer, Renate, Peyralans, Jérôme J.-P., Podtetenieff, John, Prasad, Shreenath, Soni, Pankaj, Taglieber, Andreas, Wu, Sheng, Zilly, Felipe E., Reetz, Manfred T.
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer Berlin Heidelberg 2008
Témata:
Methods
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7419347/
https://ncbi.nlm.nih.gov/pubmed/18820909
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-008-1678-9
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