Comparison of media and standards for SARS-CoV-2 RT-qPCR without prior RNA preparation

Since the emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, there have been demands on the testing infrastructure that have strained testing capacity. As a simplification of method, we confirm the efficacy of RNA extraction-free RT-qPCR and saline as an alternat...

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Veröffentlicht in:medRxiv
Hauptverfasser: Ragan, Katherine B., Bhadra, Sanchita, Choi, Joon H., Towers, Dalton, Sullivan, Christopher S., Ellington, Andrew D.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory 2020
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7418746/
https://ncbi.nlm.nih.gov/pubmed/32793925
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/2020.08.01.20166173
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