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Characterizing the Two-photon Absorption Properties of Fluorescent Molecules in the 680-1300 nm Spectral Range

Two-photon laser scanning microscopy (2PLSM) is a state-of-the-art technique used for non-invasive imaging deep inside the tissue, with high 3D resolution, minimal out-of-focus photodamage, and minimal autofluorescence background. For optimal application of fluorescent probes in 2PLSM, their two-pho...

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Publicat a:Bio Protoc
Autors principals: Drobizhev, Mikhail, Molina, Rosana S, Hughes, Thomas E
Format: Artigo
Idioma:Inglês
Publicat: Bio-Protocol 2020
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7409827/
https://ncbi.nlm.nih.gov/pubmed/32775539
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3498
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