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Efficient Nuclease-Directed Integration of Lentivirus Vectors into the Human Ribosomal DNA Locus

Lentivirus vectors (LVs) are efficient tools for gene transfer, but the non-specific nature of transgene integration by the viral integration machinery carries an inherent risk for genotoxicity. We modified the integration machinery of LVs and harnessed the cellular DNA double-strand break repair ma...

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Publicado en:Mol Ther
Main Authors: Schenkwein, Diana, Afzal, Saira, Nousiainen, Alisa, Schmidt, Manfred, Ylä-Herttuala, Seppo
Formato: Artigo
Idioma:Inglês
Publicado: American Society of Gene & Cell Therapy 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7403359/
https://ncbi.nlm.nih.gov/pubmed/32504545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymthe.2020.05.019
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