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Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry

Measuring changes in enzymatic activity over time from small numbers of cells remains a significant technical challenge. In this work, a method for sampling the cytoplasm of cells is introduced to extract enzymes and measure their activity at multiple time points. A microfluidic device, termed the L...

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Detalhes bibliográficos
Publicado no:Small
Main Authors: Mukherjee, Prithvijit, Berns, Eric J., Patino, Cesar A., Moully, Elamar Hakim, Chang, Lingqian, Nathamgari, S. Shiva P., Kessler, John A., Mrksich, Milan, Espinosa, Horacio D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2020
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7401324/
https://ncbi.nlm.nih.gov/pubmed/32452612
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/smll.202000584
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