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Expression of recombinant classical swine fever virus E2 glycoprotein by endogenous Txnip promoter in stable transgenic CHO cells

As the main immunogen that could stimulate neutralized antibody in pigs, recombinant E2 protein of CSFV was expressed in CHO‐dhfr(−)cells driven by endogenous Txnip promoter from Chinese hamster. Different fragments of Txnip promoter were amplified by PCR from isolated genomic DNA of CHO cells and c...

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Detalhes bibliográficos
Publicado no:Eng Life Sci
Main Authors: Feng, Lei, Chen, Li, Yun, Junwen, Cao, Xinglin
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7401223/
https://ncbi.nlm.nih.gov/pubmed/32774204
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elsc.201900147
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