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Fluorescence-assisted sequential insertion of transgenes (FASIT): an approach for increasing specific productivity in mammalian cells

Currently, the generation of cell lines for the production of recombinant proteins has the limitation of unstable gene expression due to the repeat-induced gene silencing or the loss of transgene copies resulting from recombination events. In this work, we developed a new strategy based on the seque...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Sci Rep
Prif Awduron: Bravo, Felipe E., Parra, Natalie C., Camacho, Frank, Acosta, Jannel, González, Alaín, Toledo, Jorge R., Sanchez, Oliberto
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Nature Publishing Group UK 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7392891/
https://ncbi.nlm.nih.gov/pubmed/32732973
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-69709-1
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