Time-saving method for directly amplifying and capturing a minimal amount of pancreatic tumor-derived mutations from fine-needle aspirates using digital PCR

It is challenging to secure a cytopathologic diagnosis using minute amounts of tumor fluids and tissue fragments. Hence, we developed a rapid, accurate, low-cost method for detecting tumor cell-derived DNA from limited amounts of specimens and samples with a low tumor cellularity, to detect KRAS mut...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Ono, Yusuke, Hayashi, Akihiro, Maeda, Chiho, Suzuki, Mayumi, Wada, Reona, Sato, Hiroki, Kawabata, Hidemasa, Okada, Tetsuhiro, Goto, Takuma, Karasaki, Hidenori, Mizukami, Yusuke, Okumura, Toshikatsu
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2020
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7378187/
https://ncbi.nlm.nih.gov/pubmed/32704002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-69221-6
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