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Improved nondegenerate multiphoton microscopy and axial registration with a reflective objective

Conventional, degenerate multiphoton microscopy (D-MPM) requires the use of a high-numerical-aperture (NA) objective. Nondegenerate MPM (ND-MPM) imposes the additional demand for precise spatiotemporal overlap of two distinct excitation sources. We demonstrate that the axial focal shift introduced b...

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Bibliografske podrobnosti
izdano v:Opt Lett
Main Authors: Hassan, Ahmed M., Engelmann, Shaun, Dunn, Andrew K.
Format: Artigo
Jezik:Inglês
Izdano: 2019
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC7370993/
https://ncbi.nlm.nih.gov/pubmed/31613252
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OL.44.005017
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