Utilizing cooled liquid chromatography and chemical derivatization to separate and quantify C3-epimers of 25-hydroxy vitamin D and low abundant 1α,25(OH)(2)D3: application in a pediatric population

There is need for a single assay able to quantify the most biologically active metabolite, 1α,25-dihydroxy-vitamin-D3, and the recently discovered biologically distinct C3-epimers of 25OHD, in addition to traditional vitamin D metabolites. We developed a method of chromatographic separation and abso...

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Detalhes bibliográficos
Publicado no:J Steroid Biochem Mol Biol
Main Authors: DeFelice, Brian C., Pedersen, Theresa L., Shorrosh, Hanan, Johnson, Randi K., Seifert, Jennifer A., Norris, Jill M., Fiehn, Oliver
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7363309/
https://ncbi.nlm.nih.gov/pubmed/31715316
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsbmb.2019.105519
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