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Positive Selection of Specific Antibodies Produced against Fusion Proteins
A method for the positive selection of specific antibodies for target proteins expressed as fusion proteins for the production of antiserum is presented. As proof of concept, the fusion protein FLAG::His::GFP::His::FLAG was expressed in Escherichia coli, purified, and used for the immunization of ra...
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| Publicado no: | Methods Protoc |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MDPI
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7359703/ https://ncbi.nlm.nih.gov/pubmed/32397084 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/mps3020037 |
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