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Assessing Budding Yeast Phosphoproteome Dynamics in a Time-Resolved Manner using TMT10plex Mass Tag Labeling

Amine-reactive Tandem Mass Tag 10plex (TMT10plex) labeling permits multiplexed protein identification and quantitative analysis by tandem mass spectrometry (MS/MS). We have used this technology to label 20 Saccharomyces cerevisiae samples collected in a time-resolved manner from a wild-type and phos...

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Detalhes bibliográficos
Publicado no:STAR Protoc
Main Authors: Jones, Andrew W., Flynn, Helen R., Uhlmann, Frank, Snijders, Ambrosius P., Touati, Sandra A.
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7357674/
https://ncbi.nlm.nih.gov/pubmed/32685930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100022
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