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The Effect of Calcium Buffering and Calcium Sensor Type on the Sensitivity of an Array-Based Bitter Receptor Screening Assay

The genetically encoded calcium sensor protein Cameleon YC3.6 has previously been applied for functional G protein–coupled receptor screening using receptor cell arrays. However, different types of sensors are available, with a wide range in [Ca(2+)] sensitivity, Hill coefficients, calcium binding d...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Chem Senses
Prif Awduron: Roelse, Margriet, Wehrens, Ron, Henquet, Maurice Gl, Witkamp, Renger F, Hall, Robert D, Jongsma, Maarten A
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Oxford University Press 2019
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7357244/
https://ncbi.nlm.nih.gov/pubmed/31278864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/chemse/bjz044
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