Efficiency and cell viability implications using tip type electroporation in zebrafish sperm cells

Sperm-mediated gene transfer (SMGT) has a potential use for zebrafish transgenesis. However, transfection into fish sperm cells still needs to be improved. The objective was to demonstrate the feasibility of tip type electroporation in zebrafish sperm, showing a protocol that provide high transfecti...

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Pubblicato in:Mol Biol Rep
Autori principali: Daneluz, Larissa O., Acosta, Izani B., Nunes, Leandro S., Blodorn, Eduardo B., Domingues, William B., Martins, Amanda W. S., Dellagostin, Eduardo N., Rassier, Gabriela T., Corcini, Carine D., Fróes, Charles N., Komninou, Eliza R., Varela, Antônio S., Campos, Vinicius F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer Netherlands 2020
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Original Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7356131/
https://ncbi.nlm.nih.gov/pubmed/32661869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11033-020-05658-2
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