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Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer
The ability to generate chimeric mice through microinjecting embryonic stem (ES) cells into blastocysts is a critical step for the conventional ES cell-mediated knockout technology. In recent years, designer nuclease-based methods, especially the CRISPR technology, have substantially decreased the n...
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| Udgivet i: | Methods Mol Biol |
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| Main Authors: | , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2019
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7354057/ https://ncbi.nlm.nih.gov/pubmed/30353510 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8831-0_6 |
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