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Chimeric Mouse Generation by ES Cell Blastocyst Microinjection and Uterine Transfer

The ability to generate chimeric mice through microinjecting embryonic stem (ES) cells into blastocysts is a critical step for the conventional ES cell-mediated knockout technology. In recent years, designer nuclease-based methods, especially the CRISPR technology, have substantially decreased the n...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Methods Mol Biol
Päätekijät: Du, Yubin, Xie, Wen, Zhang, Fan, Liu, Chengyu
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 2019
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC7354057/
https://ncbi.nlm.nih.gov/pubmed/30353510
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-8831-0_6
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