Discovery of Highly Active Recombinant PNGase H(+) Variants Through the Rational Exploration of Unstudied Acidobacterial Genomes

Peptide-N(4)-(N-acetyl-β-glucosaminyl) asparagine amidases (PNGases, N-glycanases, EC 3.5.1.52) are indispensable tools in releasing N-glycans from glycoproteins. So far, only a limited number of PNGase candidates are available for the structural analysis of glycoproteins and their glycan moieties....

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Bibliografiska uppgifter
I publikationen:Front Bioeng Biotechnol
Huvudupphovsmän: Guo, Rui-Rui, Comamala, Gerard, Yang, Huan-Huan, Gramlich, Marius, Du, Ya-Min, Wang, Ting, Zeck, Anne, Rand, Kasper Dyrberg, Liu, Li, Voglmeir, Josef
Materialtyp: Artigo
Språk:Inglês
Publicerad: Frontiers Media S.A. 2020
Ämnen:
Bioengineering and Biotechnology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC7348039/
https://ncbi.nlm.nih.gov/pubmed/32719787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.00741
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