SCAR marker for identification and discrimination of specific medicinal Lycium chinense Miller from Lycium species from ramp-PCR RAPD fragments

In the current study, ramp-PCR fragments from improved RAPD (random amplified polymorphic DNA) amplification of Lycium (Goji) species or cultivars were cut and cloned into the vector of pGEM-T. A positive clone 10–5 was screened by PCR amplification, enzymatic digestion, and Sanger sequencing. A SCA...

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Bibliografiske detaljer
Udgivet i:3 Biotech
Main Authors: Liu, Xiaoyan, Cheng, Jingliang, Mei, Zhiqiang, Wei, Chunli, Khan, Md. Asaduzzaman, Peng, Jiangzhou, Fu, Junjiang
Format: Artigo
Sprog:Inglês
Udgivet: Springer International Publishing 2020
Fag:
Short Reports
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7338294/
https://ncbi.nlm.nih.gov/pubmed/32656067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-020-02325-y
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