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Western blotting of high and low molecular weight proteins using heat
A method for the electrophoretic transfer of high and low molecular weight proteins to nitrocellulose membranes following sodium dodecyl sulfate (SDS) polyacrylamide gel is described here. The transfer was performed with heated (70-75°C) normal transfer buffer from which methanol had been omitted. C...
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| Veröffentlicht in: | Methods Mol Biol |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2015
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7337971/ https://ncbi.nlm.nih.gov/pubmed/26044007 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2694-7_26 |
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