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nanoDSF: In vitro Label-Free Method to Monitor Picornavirus Uncoating and Test Compounds Affecting Particle Stability

Thermal shift assays measure the stability of macromolecules and macromolecular assemblies as a function of temperature. The Particle Stability Thermal Release Assay (PaSTRy) of picornaviruses is based on probes becoming strongly fluorescent upon binding to hydrophobic patches of the protein capsid...

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Publicat a:Front Microbiol
Autors principals: Real-Hohn, Antonio, Groznica, Martin, Löffler, Nadine, Blaas, Dieter, Kowalski, Heinrich
Format: Artigo
Idioma:Inglês
Publicat: Frontiers Media S.A. 2020
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7333345/
https://ncbi.nlm.nih.gov/pubmed/32676065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2020.01442
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