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Clinical Validation of a SARS-CoV-2 Real-Time Reverse Transcription PCR Assay Targeting the Nucleocapsid Gene

BACKGROUND: Detection of SARS-CoV-2 viral RNA is important for the diagnosis and management of COVID-19. METHODS: We present a clinical validation of a RT-PCR assay for the SARS-CoV-2 nucleocapsid (N1) gene. Offboard lysis on an automated nucleic acid extraction system (EMAG®) was optimized with end...

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Pubblicato in:J Appl Lab Med
Autori principali: SoRelle, Jeffrey A, Frame, Ithiel, Falcon, Alejandra, Jacob, Jerin, Wagenfuehr, Jennifer, Mitui, Midori, Park, Jason Y, Filkins, Laura
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7314039/
https://ncbi.nlm.nih.gov/pubmed/32483586
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/jalm/jfaa089
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