Combined Optimization of Codon Usage and Glycine Supplementation Enhances the Extracellular Production of a β-Cyclodextrin Glycosyltransferase from Bacillus sp. NR5 UPM in Escherichia coli

Two optimization strategies, codon usage modification and glycine supplementation, were adopted to improve the extracellular production of Bacillus sp. NR5 UPM β-cyclodextrin glycosyltransferase (CGT-BS) in recombinant Escherichia coli. Several rare codons were eliminated and replaced with the ones...

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Pubblicato in:Int J Mol Sci
Autori principali: Nik-Pa, Nik Ida Mardiana, Sobri, Mohamad Farhan Mohamad, Abd-Aziz, Suraini, Ibrahim, Mohamad Faizal, Kamal Bahrin, Ezyana, Mohammed Alitheen, Noorjahan Banu, Ramli, Norhayati
Natura: Artigo
Lingua:Inglês
Pubblicazione: MDPI 2020
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7313058/
https://ncbi.nlm.nih.gov/pubmed/32486212
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21113919
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