Functional Heterologous Expression of Mature Lipase LipA from Pseudomonas aeruginosa PSA01 in Escherichia coli SHuffle and BL21 (DE3): Effect of the Expression Host on Thermal Stability and Solvent Tolerance of the Enzyme Produced

This study aimed to express heterologously the lipase LipA from Pseudomonas aeruginosa PSA01 obtained from palm fruit residues. In previous approaches, LipA was expressed in Escherichia coli fused with its signal peptide and without its disulfide bond, displaying low activity. We cloned the mature L...

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Bibliografiske detaljer
Udgivet i:Int J Mol Sci
Main Authors: Pulido, Ingrid Yamile, Prieto, Erlide, Pieffet, Gilles Paul, Méndez, Lina, Jiménez-Junca, Carlos A.
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2020
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7312249/
https://ncbi.nlm.nih.gov/pubmed/32486240
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms21113925
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