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A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

A genetic screen for mutant alleles that suppress phenotypic defects caused by a mutation is a powerful approach to identify genes that belong to closely related biochemical pathways. Previous methods such as the Synthetic Genetic Array (SGA) analysis, and random mutagenesis techniques using ultravi...

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Publicat a:J Vis Exp
Autors principals: Marayati, Bahjat F., Pease, James B., Zhang, Ke
Format: Artigo
Idioma:Inglês
Publicat: 2019
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7309966/
https://ncbi.nlm.nih.gov/pubmed/30907886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/59133
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