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A ligation and restriction enzyme independent cloning technique: an alternative to conventional methods for cloning hard-to-clone gene segments in the influenza reverse genetics system

BACKGROUND: Reverse genetics is used in many laboratories around the world and enables the creation of tailor-made influenza viruses with a desired genotype or phenotype. However, the process is not flawless, and difficulties remain during cloning of influenza gene segments into reverse genetics vec...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Virol J
Prif Awduron: Bhat, Sushant, Bialy, Dagmara, Sealy, Joshua E., Sadeyen, Jean-Remy, Chang, Pengxiang, Iqbal, Munir
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: BioMed Central 2020
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7309217/
https://ncbi.nlm.nih.gov/pubmed/32576218
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12985-020-01358-2
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