hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue

Here, we describe a new immersion-based clearing method suitable for optical clearing of thick adult human brain samples while preserving its lipids and lipophilic labels such as 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI). This clearing procedure is simple, easy to impl...

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Publicat a:Sci Rep
Autors principals: Hildebrand, Sven, Schueth, Anna, Wangenheim, Klaus von, Mattheyer, Christian, Pampaloni, Francesco, Bratzke, Hansjürgen, Roebroeck, Alard F., Galuske, Ralf A. W.
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group UK 2020
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Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7305111/
https://ncbi.nlm.nih.gov/pubmed/32561795
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-66999-3
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