Immobilization and Purification of Enzymes With the Novel Affinity Tag ChBD-AB From Chitinolyticbacter meiyuanensis SYBC-H1

A new protein immobilization and purification system has been developed based on the improved plasmid vectors, designated pETChBD-X, which contained the gene coding for two novel chitin-binding domains ChBD-AB, factor Xa cleavage site and adapted for gene fusions. The ChBD-AD from Chitinolyticbacter...

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Bibliographische Detailangaben
Veröffentlicht in:Front Bioeng Biotechnol
Hauptverfasser: Zhou, Jie, Chen, Jianhao, Zhuang, Nisha, Zhang, Alei, Chen, Kequan, Xu, Ning, Xin, Fengxue, Zhang, Wenming, Dong, Weiliang, Jiang, Min
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2020
Schlagworte:
Bioengineering and Biotechnology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7303509/
https://ncbi.nlm.nih.gov/pubmed/32596227
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2020.00579
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