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Preparation of a phosphotyrosine-protein standard for use in semiquantitative western blotting with enhanced chemiluminescence

Protein tyrosine phosphorylation is key to activation of receptor tyrosine kinases (RTK) that drive development of some cancers. One challenge of RTK-targeted therapy is identification of those tumors that express non-mutated but activated RTKs. Phosphotyrosine (pTyr) RTK levels should be more predi...

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Bibliographische Detailangaben
Veröffentlicht in:PLoS One
Hauptverfasser: Kendrick, Nancy, Powers, Ginny, Johansen, Jon, Hoelter, Matt, Koll, Andrew, Carlson, Sofia, Channaveerappa, Devika, Darie, Costel C.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2020
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7302481/
https://ncbi.nlm.nih.gov/pubmed/32555693
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0234645
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