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Massively parallel Cas13 screens reveal principles for guide RNA design
Type VI CRISPR enzymes are RNA-targeting proteins with nuclease activity that enable specific and robust target gene knock-down without altering the genome. To define rules for the design of Cas13d guide RNAs, we conducted massively-parallel screens targeting mRNAs of a green fluorescent protein (GF...
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| Udgivet i: | Nat Biotechnol |
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| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7294996/ https://ncbi.nlm.nih.gov/pubmed/32518401 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41587-020-0456-9 |
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