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Establishment and transcriptomic analyses of a cattle rumen epithelial primary cells (REPC) culture by bulk and single-cell RNA sequencing to elucidate interactions of butyrate and rumen development

As a critical and high-value tool to study the development of rumen, we established a stable rumen epithelial primary cell (REPC) culture from a two-week-old Holstein bull calf rumen epithelial tissue. The transcriptomic profiling of the REPC and the direct effects of butyrate on gene expression wer...

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Bibliographic Details
Published in:Heliyon
Main Authors: Lin, Shudai, Fang, Lingzhao, Kang, Xiaolong, Liu, Shuli, Liu, Mei, Connor, Erin E., Baldwin, Ransom L., Liu, George, Li, Cong-Jun
Format: Artigo
Language:Inglês
Published: Elsevier 2020
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7287249/
https://ncbi.nlm.nih.gov/pubmed/32551379
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.heliyon.2020.e04112
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