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Automated 3D light-sheet screening with high spatiotemporal resolution reveals mitotic phenotypes

3D cell cultures enable the in vitro study of dynamic biological processes such as the cell cycle, but their use in high-throughput screens remains impractical with conventional fluorescent microscopy. Here, we present a screening workflow for the automated evaluation of mitotic phenotypes in 3D cel...

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Detalhes bibliográficos
Publicado no:J Cell Sci
Main Authors: Eismann, Björn, Krieger, Teresa G., Beneke, Jürgen, Bulkescher, Ruben, Adam, Lukas, Erfle, Holger, Herrmann, Carl, Eils, Roland, Conrad, Christian
Formato: Artigo
Idioma:Inglês
Publicado em: The Company of Biologists Ltd 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7286290/
https://ncbi.nlm.nih.gov/pubmed/32295847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.245043
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