Development and analytical validation of real-time PCR for the detection of Streptococcus agalactiae in pregnant women

BACKGROUND: Group B Streptococcus (GBS) is the leading cause of invasive neonatal infection. In this study, we aimed to evaluate the analytical validation of qualitative real-time polymerase chain reaction (qPCR) as a means to detect GBS. METHODS: Genomic DNA (gDNA) was purified from 12 ATCC bacteri...

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Udgivet i:BMC Pregnancy Childbirth
Main Authors: Escobar, Daniel F., Diaz-Dinamarca, Diego A., Hernández, Carlos F., Soto, Daniel A., Manzo, Ricardo A., Alarcón, Pedro I., Pinto, Camila H., Bastias, Diego N., Oberg-Bravo, Carolayn N., Rojas, Robert, Illanes, Sebastián E., Kalergis, Alexis M., Vasquez, Abel E.
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2020
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7285471/
https://ncbi.nlm.nih.gov/pubmed/32517670
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12884-020-03038-z
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