Dissecting the Polyhydroxyalkanoate-Binding Domain of the PhaF Phasin: Rational Design of a Minimized Affinity Tag

Phasin PhaF from Pseudomonas putida consists of a modular protein whose N-terminal domain (BioF) has been demonstrated to be responsible for binding to the polyhydroxyalkanoate (PHA) granule. BioF has been exploited for biotechnological purposes as an affinity tag in the functionalization of PHA bea...

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Bibliographic Details
Published in:Appl Environ Microbiol
Main Authors: Mato, Aranzazu, Blanco, Francisco G., Maestro, Beatriz, Sanz, Jesús M., Pérez-Gil, Jesús, Prieto, M. Auxiliadora
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2020
Subjects:
Biotechnology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7267194/
https://ncbi.nlm.nih.gov/pubmed/32303541
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00570-20
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