Droplet digital polymerase chain reaction-based quantification of circulating microRNAs using small RNA concentration normalization

Quantification of plasma microRNAs (miRNAs) as non-invasive disease biomarkers is subject to multiple technical variabilities. This study aimed to develop an optimized protocol for miRNA quantification from rodent plasma. We hypothesized that a fixed small RNA concentration input for reverse transcr...

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Bibliographic Details
Published in:Sci Rep
Main Authors: Das Gupta, Shalini, Ndode-Ekane, Xavier Ekolle, Puhakka, Noora, Pitkänen, Asla
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2020
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7265372/
https://ncbi.nlm.nih.gov/pubmed/32488181
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-66072-z
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