Spectroscopic Characterization of Halorhodopsin Reconstituted into Nanodisks Using Native Lipids

We successfully reconstituted single Natronomonas pharaonis halorhodopsin (NpHR) trimers into a nanodisk (ND) using the native archaeal lipid (NL) and an artificial lipid having a zwitterionic headgroup, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). Incorporation of single trimeric NpHR i...

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Detalles Bibliográficos
Publicado en:Biophys J
Main Authors: Yamamoto, Ayumi, Tsukamoto, Takashi, Suzuki, Kenshiro, Hashimoto, Eri, Kobashigawa, Yoshihiro, Shibasaki, Kousuke, Uchida, Takeshi, Inagaki, Fuyuhiko, Demura, Makoto, Ishimori, Koichiro
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2020
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Articles
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7264843/
https://ncbi.nlm.nih.gov/pubmed/32396848
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2020.04.021
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